734 Innovative reconstructed atopic dermatitis model: A state-of-the-art technology to study atopic dermatitis and develop specific dermocosmetic ingredients

The skin is our interface with the world and reflects general heath state. Atopic Dermatitis (AD) an eczematous disorder affecting 15-25% of children 7-10% adult’s population. In atopic dermatitis, main characteristic a dysfunction barrier strong decrease differentiation markers including dysregulation lipids organization at lesional site. development specialized human reconstructed models very adapted tool allowing to better understand Dermatitis. We developed epidermal in which we induced characteristics AD. Indeed, 3D are using well-defined culture medium application combination cytokines glucocerebrosidase (an enzyme ceramide pathway) gene silencing on keratinocytes, order induce immune study modulation related was deepened by immunostaining models. AD were identified epidermis model. function affected these Actually, thickness tissues impacted silencing. Several proteins complex downregulated such as filaggrin, dysregulated balance lipids, cohesion also modulated. Our Atopic-dermatitis-like model specific regulators disease. This type fine-tuned evaluate disorder. kind bioengineered will be used screen identify compounds ingredients beneficial effects disturbed inflammatory signals delivered